LISTERINE® Antiseptic 30-Second Exposure

See how rinsing with 20 mL of LISTERINE® Antiseptic for 30 seconds twice a day offers maximum efficacy


For maximum efficacy, the dosing of LISTERINE® Antiseptic mouthwashes is 20 mL for 30 seconds, twice a day. But what happens in those 30 seconds, and why is 30 seconds of rinsing necessary?

In just 30 seconds of a full strength rinse, the powerful technology behind LISTERINE® Antiseptic damages the cell surface of bacteria, interferes with growth, and increases the amount of time it takes for the bacteria to regrow,1-7 as well as yields greater bacterial kill and greater plaque reduction.8*

Benefits of LISTERINE® Mouthwash
Gram Positive and Gram Negative Bacteria

Gram-Positive / Gram-Negative Bacteria

Learn about the broad spectrum of bacteria that LISTERINE® Brand rinses destroy


Formulated to provide greater fluoride uptake


*STUDY DESIGN: In this lab model, saliva-derived biofilms were grown on saliva-coated glass slides. Biofilms were cultured for 65 hours prior to mouthrinse exposure. Biofilms were stained to enable quantification of the live/dead cells.

References: 1. Fine DH, Letizia J, Mandel ID. The effect of rinsing with Listerine antiseptic on the properties of developing dental plaque. J Clin Periodontol. 1985;12:660-666. 2. Ricci-Nittel D, Fourre T. In vivo evaluation of antimicrobial activity of an essential-oil mouthrinse. Presented at: General Session of the International Association for Dental Research: March 16-19, 2011; San Diego, CA. 3. Ross NM, Charles CH, Dills SS. Long-term effects of Listerine antiseptic on dental plaque and gingivitis. J Clin Dent. 1989;1(4):92-95. 4. Drake D, Villhauer AL. An in vitro comparative study determining bactericidal activity of stabilized chlorine dioxide and other oral rinses. J Clin Dent. 2011;22(1):1-5. 5. Andreana S, Nittel-Ricci D, Wu MM, Harper DS, Baxter KA. In vitro antimicrobial activity of vanilla mint Listerine® antiseptic mouthrinse. Presented at: IADR/AADR/CADR 85th General Session and Exhibition. March 2007. 6. Pan PH, Finnegan MB, Sturdivant L, Barnett ML. Comparative antimicrobial activity of an essential oil and an amine fluoride/stannous fluoride mouthrinse in vitro. J Clin Periodontol. 1999;26(7):474-476.7. Kubert D, Rubin M, Barnett ML, Vincent JW. Antiseptic mouthrinse-induced microbial cell surface alterations. Am J Dent. 1993;6(6):277-279. 8. Fine DH, Furgang D, Barnett ML, et al. Effect of an essential oil-containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels. J Clin Periodontol. 2000;27(3):157-161.